Chrysalin (TP-508)

Overview of Chrysalin (TP-508) Black Tops

RESEARCH USE ONLY: This product is intended for laboratory research purposes only. Not for human or animal consumption.

Chrysalin (TP-508) is also known as rusalatide acetate. It is a synthetic 23-amino acid peptide. It represents residues 508–530 of prothrombin. This is specifically true for the high-affinity receptor-binding domain of the native thrombin molecule. It is a nonproteolytic thrombin peptide. This basically means that it does not activate the proteolytic cleavage cascade associated with thrombin’s coagulation function.

In laboratory settings, TP-508 is checked for how it interacts with a distinct subset of thrombin receptors on:

• Fibroblasts,
• Endothelial cells, and 
• Stem cells

TP-508 is not a SARM, not a hormone analogue, and not a dietary supplement. RESEARCH USE ONLY: This product is intended for laboratory research purposes only. Not for human consumption.

Chemical and Molecular Properties

Property	Value
CAS Number	121341-81-9
Molecular Formula	C₉₇H₁₄₆N₂₈O₃₆S
Molecular Weight	2,312.44 g/mol
IUPAC Name	H-Ala-Gly-Tyr-Lys-Pro-Asp-Glu-Gly-Lys-Arg-Gly-Asp-Ala-Cys-Glu-Gly-Asp-Ser-Gly-Gly-Pro-Phe-Val-OH
PubChem CID	91668177
Peptide Length	23 amino acids
Sequence	Ala-Gly-Tyr-Lys-Pro-Asp-Glu-Gly-Lys-Arg-Gly-Asp-Ala-Cys-Glu-Gly-Asp-Ser-Gly-Gly-Pro-Phe-Val
Peptide Source	Residues 508–530 of human prothrombin
Purity	≥98% (HPLC verified) — per supplier COA
Physical Form	White to off-white lyophilised powder
Vial Format	Black top lyophilised vial (flip-top aluminium crimp cap, sterile sealed)
Solubility	Soluble in sterile water or 0.1% acetic acid solution
Storage	Store at −20°C, protected from light and moisture
Synonyms	TP508, Chrysalin®, rusalatide acetate, TRAP-508
Classification	Research Use Only (RUO)

Formulation note for researchers: 

Chrysalin (TP-508) is supplied as a lyophilised powder in a black top vial. This is a sterile, sealed glass vial with a black flip-top aluminium crimp cap. This format maintains peptide integrity under recommended cold storage conditions and is standard for research-grade lyophilised peptides. The acetate salt form is the standard supplied form for most research applications. Each PureRawz batch comes with a Certificate of Analysis. The CoA confirms the specific molecular identity and form of the shipped material. 

Mechanisms Investigated in Preclinical Research

Nonproteolytic Thrombin Receptor Binding and eNOS Activation Research: 

TP-508 has been investigated in preclinical cell culture systems as a nonproteolytic agonist. This is true at a distinct subset of thrombin receptors expressed on fibroblasts and endothelial cells. In isolated endothelial cell systems, TP-508 has been observed to stimulate endothelial nitric oxide synthase (eNOS) activation and rapid NO production 

Angiogenic Signalling and Endothelial Cell Motility Research: 

In preclinical animal wound healing models, TP-508 has been observed to stimulate endothelial cell chemokinesis and chemotaxis. This obviously happens in a dose-dependent manner. In rodent models, increased vascularisation markers at wound sites have been observed in TP-508-treated groups compared to saline controls. The αvβ3 integrin pathway has been identified as one mechanism through which TP-508 may affect endothelial signal transduction in these experimental settings. 

Fibroblast Gene Expression Pathway Research: 

In isolated fibroblast cell systems, TP-508 has been investigated for its potential to modulate differential gene expression. This is believed to occur through a nonproteolytic receptor activation pathway. Preclinical data suggest this may involve upstream signalling events distinct from those initiated by proteolytically active thrombin. 

Stem Cell Proliferation Pathway Research: 

In in vitro model systems, TP-508 has been investigated for its potential to affect stem cell proliferation signalling. Preclinical data from adipose tissue-derived stem cell culture suggest TP-508 may interact with PI3 kinase/Akt pathway activity. 

Research Applications

Nonproteolytic thrombin receptor binding kinetics and structure-activity relationship (SAR) studies

1. eNOS activation and nitric oxide signalling pathway research
2. Angiogenesis assay reference compound 
3. αvβ3 integrin-mediated signal transduction research 
4. Fibroblast gene expression pathway investigation 
5. Comparative thrombin fragment pharmacology studies

Risk & Handling

Handling Precautions: 

Chrysalin (TP-508) should be handled exclusively in a controlled laboratory environment by trained personnel. Use appropriate PPE at all times. Avoid direct skin and eye contact during reconstitution. Avoid inhalation of powder during weighing or handling of the lyophilised form.

Exposure Risks: Risk tier: MODERATE. 

TP-508 is a nonproteolytic synthetic thrombin peptide. No human safety data has been established for the research-grade formulation supplied here. Long-term or chronic toxicity data do not exist for this research preparation. 

Storage: Store lyophilised TP-508 at −20°C in a dry, dark environment. Protect from light, heat, and moisture at all times. Avoid repeated freeze-thaw cycles. The black top vial seal should remain intact until use. Do not remove or compromise the crimp cap before reconstitution. 

Quality Assurance

• Identity: Confirmed by HPLC retention time and mass spectrometry against CAS 121341-81-9 reference data
• Purity: ≥98% (HPLC verified)
• Sequence Verification: Amino acid sequence AGYKPDEGKRGDACEGDSGGPFV confirmed against prothrombin residues 508–530
• Batch Traceability: Unique lot numbers for every product
• Certificate of Analysis (COA): Available for every batch. This confirms molecular identity, purity, and formulation
• Third-Party Testing: Independent laboratory verification
• Safety Data Sheet (SDS): Available upon request

Why PureRawz

PureRawz supplies research-grade teriparatide with batch-specific Certificates of Analysis. This confirms molecular identity and purity against CAS 121341-81-9 reference data. Given that Chrysalin is commercially available in both free peptide and acetate salt forms with different molecular weights, batch-specific identity confirmation is a key parameter for reproducible experimental design. Each lot is characterised by HPLC retention time and mass spectrometry to confirm the specific form of the shipped material.

References

1. Olszewska-Pazdrak B, Hart-Vantassell A, Carney DH. Thrombin peptide TP508 stimulates rapid nitric oxide production in human endothelial cells. Journal of Vascular Research. 2010;47(3):203–213. https://pubmed.ncbi.nlm.nih.gov/19893317/

 

1. Hembrough TA, Swartz GM, Papathanassiu A, et al. Thrombin peptide, TP508, stimulates angiogenic responses in animal models of dermal wound healing, in chick chorioallantoic membranes, and in cultured human aortic and microvascular endothelial cells. Journal of Vascular Research. 2001. https://pubmed.ncbi.nlm.nih.gov/11888680/

Disclaimer: Preclinical and published scientific research indicate potential mechanisms of action for this compound in laboratory and animal models. Any references to published studies are for informational purposes only and do not constitute a claim that this product produces the same effects in humans. The safety and efficacy of this compound in humans have not been established for the research-grade formulation supplied here.

ATTENTION: All products sold by PureRawz are intended for laboratory and research purposes only and are not approved for human consumption, veterinary use, or any other use. Products are not evaluated by the FDA and are not intended to diagnose, treat, cure, or prevent any disease. Customers assume full responsibility for the lawful use of these products in accordance with all applicable federal, state, and local regulations.